automatic amino acid analyzer a300-advanced Search Results


tms a300  (Malvern Panalytical)
99
Malvern Panalytical tms a300
Tms A300, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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automatic amino acid analyzer membrapure a300 advanced  (membraPure gmbh)
90
membraPure gmbh automatic amino acid analyzer membrapure a300 advanced
Automatic Amino Acid Analyzer Membrapure A300 Advanced, supplied by membraPure gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycn phospho ser54  (Bethyl)
93
Bethyl mycn phospho ser54
Mycn Phospho Ser54, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gapdh fitzerald industries international  (Bethyl)
96
Bethyl gapdh fitzerald industries international
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Gapdh Fitzerald Industries International, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti bclaf  (Bethyl)
93
Bethyl anti bclaf
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Anti Bclaf, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal antibodies  (Bethyl)
96
Bethyl polyclonal antibodies
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Polyclonal Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti kap1  (Bethyl)
94
Bethyl anti kap1
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Anti Kap1, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti ureb1 lasu1  (Bethyl)
93
Bethyl anti ureb1 lasu1
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Anti Ureb1 Lasu1, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smc3  (Bethyl)
95
Bethyl smc3
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Smc3, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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taf15  (Bethyl)
94
Bethyl taf15
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Taf15, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtr4  (Bethyl)
92
Bethyl mtr4
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Mtr4, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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afm a300  (Angstrom Advanced Inc)
90
Angstrom Advanced Inc afm a300
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Afm A300, supplied by Angstrom Advanced Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; RPA32, the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).

Journal: DNA repair

Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

doi: 10.1016/j.dnarep.2018.12.001

Figure Lengend Snippet: Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; RPA32, the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).

Article Snippet: Additional antibodies used in this study: table ft1 table-wrap mode="anchored" t5 Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

Techniques: Infection, Labeling, Immunoprecipitation, Western Blot, Binding Assay, Virus, Phospho-proteomics, Two-Dimensional Gel Electrophoresis, SDS Page, Incubation, DNA Sequencing, Derivative Assay

Variation in TP-oligo size during dl1004 vs. WT Ad5 infection. HeLa cells were infected with dl1004 (A) or WT (B), harvested at the indicated time post infection, and TP was immunoprecipitated with the indicated antibody before being labeled with [α-32P]-cordycepin triphosphate by TdT. Nbs1, Nijmegen breakage syndrome protein 1 (Nibrin); DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h.p.i., hours post infection.

Journal: DNA repair

Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

doi: 10.1016/j.dnarep.2018.12.001

Figure Lengend Snippet: Variation in TP-oligo size during dl1004 vs. WT Ad5 infection. HeLa cells were infected with dl1004 (A) or WT (B), harvested at the indicated time post infection, and TP was immunoprecipitated with the indicated antibody before being labeled with [α-32P]-cordycepin triphosphate by TdT. Nbs1, Nijmegen breakage syndrome protein 1 (Nibrin); DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h.p.i., hours post infection.

Article Snippet: Additional antibodies used in this study: table ft1 table-wrap mode="anchored" t5 Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

Techniques: Infection, Immunoprecipitation, Labeling, Binding Assay

2.1. Antibodies

Journal: DNA repair

Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

doi: 10.1016/j.dnarep.2018.12.001

Figure Lengend Snippet: 2.1. Antibodies

Article Snippet: Additional antibodies used in this study: table ft1 table-wrap mode="anchored" t5 Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

Techniques: